Figure 1: Parkin is physiologically sulfhydrated.
From: Sulfhydration mediates neuroprotective actions of parkin
(a) Parkin expressed in HEK293 cells is sulfhydrated by the H2S donor NaHS as detected by the modified biotin switch method. (b) Endogenous parkin is basally sulfhydrated in both mouse brain and rat striatum as detected by the maleimide assay, in which loss of red fluorescence signal following DTT treatment indicates sulfhydration of the protein. (c) Sulfhydration of myc-parkin overexpressed in HEK293 cells is enhanced almost 20-fold on overexpression of GST–CBS, one of the principle H2S producers in the brain. n=3, **P<0.01 via one-way analysis of variance (ANOVA). (d) Endogenous parkin sulfhydration in SH-SY5Y cells is enhanced over fivefold on treatment with 100 μM GYY4137, a hydrogen sulfide donor. n=3, **P<0.01 via one-way ANOVA. (e) Endogenous parkin sulfhydration in SH-SY5Y cells is enhanced over eightfold by overexpression of GST–CBS. n=3, **P<0.01 via one-way ANOVA. All data are expressed as mean±s.e.m.
