Figure 1: Specifications of experimental setup.
From: Biased Brownian stepping rotation of FoF1-ATP synthase driven by proton motive force
(a) Schematic model of the experimental setup. Lasers of 532 and 404 nm were introduced. The thickness of Ni-NTA agarose layer is around 200 nm. The depth of evanescent field is around 70 nm. (b) Fluorescence images of pHrodo (λex: 532 nm, λem: 575 nm) before (upper) and after (lower) ultraviolet light irradiation. The supported membrane is present in the white area. Scale bars: 2 μm. (c) Time course of the fluorescence intensity of pHrodo in the presence (red) or absence (blue) of the supported membrane. Error bars represent the s.d.’s of five measurements. The purple area represents the time period of ultraviolet light irradiation.
