Figure 2: Rotary motion of FoF1 in the presence of ΔpH.
From: Biased Brownian stepping rotation of FoF1-ATP synthase driven by proton motive force
(a) Sequential images of a rotating particle in the presence of ΔpH (lower). Blue and red points represent the centre of the rotation and the centroid of the gold nanoparticle, respectively. The angular positions of the particle are shown in the upper figures. The interval between images is 5 ms. Time course of the rotation of FoF1 in the presence (b) or absence (c) of nucleotides. The purple area (t>0 s) represents the time period of ultraviolet light irradiation. The solid orange lines represent the averaged time course from 17–18 molecules. (d) Histogram of the rotational speed in the absence (red) or presence (blue) of nucleotides. (e) Rotational speed plotted against the relative increment of the fluorescence of pHrodo, RI (or ΔpH, see Supplementary Fig. S1).
