Figure 3: Stepping rotation of FoF1.
From: Biased Brownian stepping rotation of FoF1-ATP synthase driven by proton motive force
(a) Time course of the stepping rotation of FoF1 in the presence of 50 μM ADP, 1 μM ATP and 200 μM Pi before (red) and after (blue) ultraviolet light irradiation. The left inset presents an enlarged view of the time course. Orange and light blue points represent the pauses before CW and CCW steps, respectively. The right inset is the centroid trajectory of the rotating particle during ultraviolet light irradiation. (b) Histograms of the angular positions before (upper) or after (lower) ultraviolet light irradiation in the presence of 50 μM ADP, 1 μM ATP and 200 μM Pi. (c) Histogram of the pairwise distribution of the angular displacement derived from the time courses of the rotation in the presence of 50 μM ADP, 1 μM ATP and 200 μM Pi. The histogram is derived from 42 rotations of three molecules. The window size of the analysis is Δt=0.5–1,000 ms.
