Figure 3: Inducible expression of Klf5 in vivo induces increased adhesion to fibronectin and normal BM homing. | Nature Communications

Figure 3: Inducible expression of Klf5 in vivo induces increased adhesion to fibronectin and normal BM homing.

From: Klf5 controls bone marrow homing of stem cells and progenitors through Rab5-mediated β1/β2-integrin trafficking

Figure 3

(a,b) Klf5 expression in LSK BM cells isolated from tet-off, Scl-tTA/WT (WT) or Scl-tTA/TRE-Klf5 mice. (a) Protein was measured by intracellular flow cytometry on BM LSK cells from Scl-tTA/TRE-Klf5 mice collected on days 2, 5 and 7 post doxycycline withdrawal. N=3 independent animals per group. Data represent mean±s.e.m. **P=0.001, ***P=0.0006, Student t-test. (b) mRNA on sorted BM LSK cells after 5 days post doxycycline withdrawal. ***P=0.0002, Student t-test. (c) Adhesion of LSK BM cells to fibronectin from tet-off, WT or Scl-tTA/Tre-Klf5 mice. *P=0.019, Student t-test. (d) Chemotaxis of LSK BM cells to CXCL12 from tet-off, WT or Scl-tTA/Tre-Klf5 mice. Data are presented as mean±s.d. (e) Homing of WT and Klf5-overexpressing HSC. Graph depicts the homing (%) after 3 h post transplantation of competitive repopulation units (CRU) analyzed as engrafting units after co-transplantation with CD45.1+ competitor BM cells into congenic recipients, analyzed at 4, 8, 12, 16 and 24 weeks post transplantation. N=8 to 10 mice per group, from two independent experiments.

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