Figure 4: Dissociated cortical progenitor cells display MST and horizontal spindle orientation.
From: Mitotic spindle orientation predicts outer radial glial cell generation in human neocortex
(a) oRG-like cell in dissociated culture undergoes MST followed by division with horizontal cleavage angle. Dissociated cortical cells were infected with low-titre GFP adenovirus or retrovirus and grown in culture for up to 5 weeks. Transmitted light microscopy allowed visualization of condensed chromatin for quantification of spindle orientation. Scale bar, 10 μm. (b) Multiple IP-like cell divisions in culture, which contrast with oRG-like divisions by their lack of MST. Individual multipolar cells were followed (blue, red and white arrowheads), and over a period of less than 5 h, each cell underwent mitosis (dotted circle) without MST, producing two multipolar daughter cells. Scale bar, 50 μm.
