Figure 6: Asymmetrical increase of synapse numbers in CA1 s.r. in ENR.
From: Experience enhances gamma oscillations and interhemispheric asymmetry in the hippocampus
(a) Immunostaining of glutamatergic presynaptic terminals by VGLUT1. Typical images of VGLUT1 immunostaining (upper panel). VGLUT1 was used to label excitatory presynaptic terminals. Each image is a maximum intensity projection of seven consecutive depth images (150 μm interval). Note that VGLUT1 immunoreactivity is more intense in the s.r. of the ENR rat. Scale bar, 10 μm. (b) Bar graphs of the left versus right fluorescence intensity balance for VGLUT1. The mean VGLUT1 fluorescence intensity was more intense in the CA1 s.r. of the ENR rat (*P<0.05, Tukey’s honestly significant difference test, NISO=4, NENR=6 (s.o.); NISO=5, NENR=9 (s.r.); NISO=5, NENR=5 (s.l-m.)). (c) Representative EM images of the left and right CA1 s.r. prepared from ISO and ENR rats. Scale bar, 1.0 μm. Arrowheads indicate the locations of postsynaptic density thickening. (d) Pairwise comparisons of left versus right spine density in CA1 s.r. Three rats were investigated for each rearing condition (**P<0.01, paired t-test, NISO=3, NENR=3). Error bars represent s.e.m.
