Figure 3: Colonic EC hyperproliferation is induced by Lactobacillus murinus.
From: Microbiota-derived lactate accelerates colon epithelial cell turnover in starvation-refed mice
(a) Number of bacteria determined by the 16S rRNA value. Mice were starved for 36 h and refed with the indicated formula in parentheses for the indicated time duration. Data are shown as an average+s.d. for three mice for each group. (b) FISH analysis of the starvation-refed colon with the 16S RNA probe. Scale bar, 100 μm. (c) DGGE analysis of colonic luminal contents in control mice and mice starved for 36 h and refed with CE2 or ORS for 2 or 24 h. Colonic luminal contents were collected and the microbial species determined, as described in the Methods. Each lane represents samples from individual mice. (d) Gnotobiotic mice were generated by inoculation of Lactobacillus murinus into germ-free mice. Frozen sections were stained by anti-BrdU antibody (green). Scale bar, 200 μm. (e) Number of BrdU+ cells is shown as relative to control gnotobiotic mice. Data are shown as an average+s.d. for three mice for each group.
