Figure 2: 8-pCPT enhances rapidly adapting mechanically evoked currents in large diameter sensory neurons.

(a) Stimulus–response curve of rapidly adapting currents evoked by mechanical stimulation before and 15 min after 8-pCPT or vehicle administration (vehicle, n=13; 8-pCPT, n=17). (b) Time course of peak currents evoked by a ~12.5-μm membrane deflection after administration of 8-pCPT into the bath solution. Repeated measures one-way analysis of variance: time, P<0.05; treatment, P>.05; interaction: P<0.01 (n=14). (c) Threshold of activation was determined as mechanical stimulus that elicited a current >20 pA. The decrease in threshold of activation after 8-pCPT was calculated as percentage of baseline thresholds. (d) Representative example of whole-cell voltage clamp traces from a large diameter mouse DRG neuron with a narrow action potential width in response to increasing membrane deformation (holding potential −60 mV) before and after 8-pCPT or vehicle administration. All data are expressed as mean±s.e.m. (a–b) Data were analysed using two-way analysis of variance followed by the Bonferroni post hoc test. (d) Data are analysed by t-test. *P<0.05, **P<0.01, ***P<0.001.