Figure 4: Y26 phosphorylation may release E19 from blocking the active site of PGAM1. | Nature Communications

Figure 4: Y26 phosphorylation may release E19 from blocking the active site of PGAM1.

From: Tyr26 phosphorylation of PGAM1 provides a metabolic advantage to tumours by stabilizing the active conformation

Figure 4

(a) Superposition of PGAM1 WT (cyan) and H11-phosphorylated form (green). Phosphorylated H11 is shown in stick and loop 12–23 with the large conformational change is marked. (b) Comparison of Y26 surrounding residues between WT PGAM1 (cyan) and H11-phosphorylated form (green). (c) Interactions between E19 and adjacent residues in Y26 buried WT PGAM1. (d) Interactions between phosphorylated H11 and adjacent residues in Y26 exposed phosphorylated PGAM1. (e) Superposed cofactor 2,3-BPG in the active site of non-phosphorylated PGAM1. There is a clash between E19 and phosphate group of 2,3-BPG. (f) Superposed substrate 3-PG in active site of H11-phosphorylated PGAM1 showing that it is ready to react with phosphorylated H11.

Back to article page