Figure 8: The expression of Max and G9a in PGCs.
From: Max is a repressor of germ cell-related gene expression in mouse embryonic stem cells
(a) Relative expression levels of Max in purified GFP-positive PGCs and in the adjacent GFP-negative somatic cells from mil1-GFP Tg embryos at different embryonic stages compared with its expression in E14-1 ES cells, which was set as 1.0. Error bars, s.d. (n=3) of biological replicates. (b) Immunostaining of E10.5 PGCs for G9a (magenta). PGCs were detected by the expression of mil1-GFP transgene (green), shown in the merged images in the right panels. G9a expression was undetectable or very low in GFP-expressing PGCs. Scale bar, 10 μm.
