Figure 1: Effect of B. subtilis VOCs on E. coli gene expression.
From: Interspecific bacterial sensing through airborne signals modulates locomotion and drug resistance
(a) Schematic showing the two-compartment plate assay (I-plate system). For growth of E. coli, LB agar was used; for visualization and photography, MacConkey-agar plate was used. (b) Relative gene-expression analysis of E. coli. Three genes showing >3-fold changes in expression in response to B. subtilis VOCs in microarray data are represented as ‘Expression level’; these were randomly selected and analysed by RT–qPCR after E. coli cells were treated with VOCs for 12 h. Error bars are defined as s.e.m. (n=3, P=0.05). (c) Clustering of microarray data. The microarray data show >3-fold up- or downregulation induced by treatment with VOCs. Clustering analysis was performed using MultiExperiment Viewer45. Three treatment periods are shown (6, 12 and 24 h) (d) Gene-expression pattern of clustered groups. Two genes were randomly selected from each of the groups in c and examined by RT–qPCR using gene-specific primers listed in Supplementary Table S6. Error bars are defined as s.e.m. (n=3, P=0.05).
