Figure 5: Master regulation of VOC-mediated phenotypes by the ypdB gene product.
From: Interspecific bacterial sensing through airborne signals modulates locomotion and drug resistance
(a) Identification of VOC-regulated genes involved in antibiotic resistance. Survival ratio of VOC-treated to VOC-untreated Keio collection mutants grown in the presence of ampicillin (n=3). Asterisk (*) indicates the statistical significance (P=0.05). (b) Growth phenotypes of wild-type and ypdB knockout by VOCs. The growth (OD600) was monitored by the absence or presence of VOCs by natural vaporization. Comparison of the effect of 2,3-BD and GA on swarming motility (c) and antibiotic resistance (d) in a ypdB-knockout and wild-type E. coli. (e) Survival ratio of VOC-treated to VOC-untreated cells in a ypdB knockout, the complemented knockout and wild-type cells grown in the presence of ampicillin. (f) Relative change in hipA expression with and without VOC treatment in wild-type E. coli and ypdB-knockout cells (n=3, P=0.05). (g) Model for VOC-mediated changes to E. coli physiology. The model is based on data from this study and previous reports. Error bars are defined as s.e.m.
