Figure 5: Expression of retinal transcription factors in Rorb1g/1g mice. | Nature Communications

Figure 5: Expression of retinal transcription factors in Rorb1g/1g mice.

From: An isoform of retinoid-related orphan receptor β directs differentiation of retinal amacrine and horizontal interneurons

Figure 5

(a) Transcription factor expression analysed by in situ hybridization (Foxn4, Ptf1a, Nd1, Nd4, Nr4a2, Ebf3) or immunohistochemistry (Prox1, bHLHb5), at stages when each factor normally displays prominent expression (early stages, left, or later maturation stages, right). Ptf1a was overtly and Nd1 and Nd4 were modestly reduced in 1g/1g mice. Expression of horizontal (Prox1) and amacrine sub-lineage (Nr4a2, bHLHb5, Ebf3) markers was severely reduced in 1g/1g mice. Scale bar, 30 μm. (b) qPCR analysis demonstrating normal Foxn4 but reduced Ptf1a mRNA levels in Rorb1g/1g mice during development. Each point represents mean of three analyses on pooled retina (>4 mice) at each age. Plots based on mean±s.d. at each age.

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