Figure 1: Oxidative demethylation of m6A in RNA by FTO.
From: FTO-mediated formation of N6-hydroxymethyladenosine and N6-formyladenosine in mammalian RNA
(a) FTO demethylates m6A through oxidization of m6A to hm6A with f6A as a further oxidized product. (b) MALDI-TOF analysis of m6A-RNA after reacting with FTO. An unexpected monoisotopic peak of m6A+14 Da corresponding to f6A was observed after treating a 9mer m6A-RNA oligo (5 μM) with 10 μM of FTO at room temperature (RT) for 20 min. The m6A−2 Da peak represents the dehydration product of the putative hm6A intermediate during MALDI-TOF ionization.
