Figure 4: Presence of hm6A and f6A in mammalian mRNA.
From: FTO-mediated formation of N6-hydroxymethyladenosine and N6-formyladenosine in mammalian RNA
(a) Detection strategy: RNA is first digested with RNase T1 to expose the 5′ OH group of the base after G. Subsequent nuclease P1 digestion releases the first base as nucleoside, and the rest of the bases as nucleoside 5′-monophosphate (5′-NMP). (b,c) LC-MS/MS analysis of digested mRNA isolated from HeLa cell and mouse liver samples showing the presence of hm6A (b, 298.1→136.0, 3.1 min) and f6A (c, 296.1→164.1, 4.2 min).
