Figure 4: TRM proteins target TTP proteins to the microtubular cytoskeleton.
From: A protein phosphatase 2A complex spatially controls plant cell division
(a–c) TON1-FASS (a) and FASS-PP2AA1 (c) BiFC signals, as well as PP2AA1-GFP (b) fluorescence all localized to the cytoplasm when expressed in N. benthamiana leaf epidermal cells. (d–f) Upon co-expression with RFP-TRM1, which labels the cortical microtubular cytoskeletal array (middle panels, red), the GFP-FASS signal (d), the BiFC TON1-FASS fluorescence (e) or the BiFC FASS-PP2AA1 fluorescence (f) relocalized to microtubule arrays where it co-localized as a punctuate staining with TRM1. (g) The BiFC TON1-FASS fluorescence also relocalized to microtubule arrays upon co-expression with RFP-TRM8. All micrographs are projections of Z-stack confocal images. Scale bars, 20 μm (a–g).
