Figure 5: TON1 and FASS and PP2AA1 all localize to the PPB.
From: A protein phosphatase 2A complex spatially controls plant cell division
(a) Time-lapse analysis of root tip cells expressing both Pro35S:GFP-FASS and H2AX-RFP showed accumulation of FASS at the cortical division zone during PPB formation, and persistence of the signal beyond PPB degradation up to the metaphase stage. H2AX-RFP served as marker for the different cell-cycle phases. Arrowheads indicate PPB and cortical division zone localization of FASS up to metaphase in cell #1. Note persistence of the signal in cell #2 at t=17 min (asterisks), excluding photobleaching-based disappearance of GFP-FASS signal in cell #1. (b) Time-lapse analysis of a root tip cell expressing both Pro35S:GFP-TON1 and H2AX-RFP, showing accumulation of TON1 at the PPB and persistence of the signal at the cortical division zone up to metaphase well after PPB disassembly. (c) Transverse (left) and tangential (right) views of a root tip cell at the preprophase stage expressing ProPP2AA1:YFP-PP2AA1 (green)27 and mCherry-β-tubulin6 (red). YFP-PP2AA1 is enriched at the PPB (arrowheads) as also evidenced by the measurement of the fluorescence intensity along the plasma membrane indicated by brackets in transverse views (green curve corresponds to the YFP-PP2AA1 fluorescence and red curve to the mCherry-β-tubulin6 signal). Scale bars, 10 μm (a–c).
