Figure 4: The minimum in the PVAV mutant 2D potential of mean force (PMF) is shifted compared with wild-type.
From: The pore of voltage-gated potassium ion channels is strained when closed
(a) The position of the minimum in the 2D PMF for the PVAV mutant Kv1.2CH is shifted towards KcsA compared with the wild type. As in the wild-type 2D PMF, the last 3 ns of each umbrella simulations is used. The positions of the equivalent residues in the structures of Kv1.2 (ref.3), closed KcsA (ref.2) and MlotiK1 (ref.60) are also plotted. Six reference points are labelled. Contours are drawn every 1 kcal mol−1. (b) The conformation of the S6 helices (in red) when viewed intracellularly.
