Figure 4: M-CSF is required for tissue-resident macrophage proliferation. | Nature Communications

Figure 4: M-CSF is required for tissue-resident macrophage proliferation.

From: Distinct bone marrow-derived and tissue-resident macrophage lineages proliferate at key stages during inflammation

Figure 4

(a) Data showing proliferation of Res MØ (gated by their F4/80highCD11bhigh phenotype), 24 h after i.p. administration of 0.4 μg of recombinant M-CSF (0.4) or carrier control (0). Data shown represent mean±s.e.m. 10 mice per group (7-week-old female C57BL/6) pooled from two identical experiments. Data were analysed by a Student’s t-test. (b) Representative flow-cytometric plots showing the identification of Res MØ in a similar way as previously described 48 h after the i.p. injection of 2 × 106 zymosan particles in conjunction with 0.5 mg of either anti-M-CSF (clone 5A1) or and rat IgG isotype control (as indicated). Percentages shown are mean values from the analyses of groups of mice (shown below). (c) Measurement of proliferation in Res MØ 48 h after i.p. injection of 2 × 106 zymosan particles in conjunction with 0.5 mg of either anti-M-CSF (clone 5A1) or and rat IgG isotype control. Data shown represent mean±s.e.m. 5 mice per group (7-week-old female C57BL/6) from one of two similar experiments. Data were analyzed by a Student’s t-test, *P<0.05, ***P<0.001.

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