Figure 1: Defective heterochromatin repair in HGPS fibroblasts. | Nature Communications

Figure 1: Defective heterochromatin repair in HGPS fibroblasts.

From: Depleting the methyltransferase Suv39h1 improves DNA repair and extends lifespan in a progeria mouse model

Figure 1

(a) Representative photos of immunofluorescence staining of H3K9me3 and γ–H2AX in HGPS dermal fibroblast cell line HGADFN003 (HG003) at 24 h after γ-irradiation (5 Gy). Scale bar, 5 μm. (b) Line scans of H3K9me3, γ–H2AX and DAPI signal intensity in the cell shown in (a). Scale bar, 5 μm. (c) Representative photos of immunofluorescence staining of H3K9me3 and γ-H2AX in SUV39 siRNA or scramble-treated HG003 and healthy control cells (PH) at 24 h after γ-irradiation (5 Gy). Scale bar, 10 μm. (d) Quantification of γ-H2AX foci in the experiment of c. At least 100 cells were counted. Data represent mean±s.e.m., *P<0.05, two-tailed t-test. (e) Representative immunoblots showing levels of H3K9me3 and γ-H2AX in PH and HG003 cells (passage 16) treated with SUV39 siRNA or scramble at 24 h after γ-irradiation (5 Gy).

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