Figure 2: Increased levels of Suv39h1 and H3K9me3 in progeroid cells. | Nature Communications

Figure 2: Increased levels of Suv39h1 and H3K9me3 in progeroid cells.

From: Depleting the methyltransferase Suv39h1 improves DNA repair and extends lifespan in a progeria mouse model

Figure 2

(a) Representative immunoblots showing the level of SUV39H1 in HEK293 cells expressing ectopic lamin A or progerin. (b) Quantification of experiments in a. Data represent mean±s.e.m., n=3. *P<0.05, two-tailed t-test. (c) Representative western blotting showing the level of Suv39h1 in wild-type and Zmpste24−/− MEFs and in kidney from wild-type and Zmpste24−/− mice. (d) Quantification of experiments in c. Data represent mean±s.e.m., n=3. *P<0.05, two-tailed t-test. (e) Representative immunoblots showing levels of different histone modifications in total cell lysate from wild-type and Zmpste24−/− MEFs and indicated wild-type and Zmpste24−/−tissues. (f) Quantification of experiments in e. Data represent mean±s.e.m., n =3. *P<0.05, two-tailed t-test. (g) Representative photos of immunofluorescence staining of H3K9me3 in wild-type and Zmpste24−/− MEFs. Scale bar, 20 μm.

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