Figure 4: Heterochromatic histone modifications in HGPS cells. | Nature Communications

Figure 4: Heterochromatic histone modifications in HGPS cells.

From: Depleting the methyltransferase Suv39h1 improves DNA repair and extends lifespan in a progeria mouse model

Figure 4

(a) Representative western blotting showing levels of heterochromatic H3K9me3 and H4K20me3 in HGPS dermal fibroblast cell lines HGADFN122 (HG122), HGADFN143 (HG143), HGADFN155 (HG155), HGADFN164 (HG164), HGADFN169 (HG169) and HGADFN188 (HG188) derived from six individuals and aged from 2 to 8 years, PH and human fibroblasts from a 5-month foetus (F2-S). All cell lines were at passage 12. (b) Relative levels of histone modifications in various cells shown in a. Data represent mean±s.e.m., n=3. *P<0.05, **P<0.01, two-tailed t-test, HGPS versus PH and F2-S versus PH. (c) Representative western blotting of H3K9me3 and H4K20me3 in human dermal fibroblasts HGADFN003 (HG003) derived from a 2-year-old male HGPS patient and PH cells from a healthy 7-month-old male at different passages. (d) Quantification of levels of histone modifications in experiments in c. Data represent mean±s.e.m., n=3. **P<0.01, two-tailed t-test, HG003 (P24) versus PH (P24). (e) Immunofluorescence staining of H3K9me3 in PH and HG003 cells. Arrows show H3K9me3-enriched nuclear compartment. Scale bar, 40 μm.

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