Figure 2: Both hematopoietic and non-hematopoietic cells take up GNVs.
From: Delivery of therapeutic agents by nanoparticles made of grapefruit-derived lipids
(a) Confocal images (top) mouse and FACS quantitative analysis (bottom) of PKH26-labelled GNVs taken up by non-hematopoietic cells (A549, GL26, 4T1, SW620 and CT26) and primary splenic lymphocytes (T and B cells). (b) Temperature (T), (c) time (n=3) and (d) concentration dependence on the efficiency of GNVs uptake (n=3). (e) Potential pathways utilized by GNVs to enter A549 cells. (f) Stability of cationic DOTAP:DOPE liposomes (top) and GNVs (bottom) at 37 °C in the presence of 10% FBS. ***P<0.001 (Student’s t-test). Data (a–e) are the mean±s.e.m. of at least five independent experiments.
