Figure 1: Casp6 activation in axons is selective to NGF deprivation.
From: Distinct pathways mediate axon degeneration during apoptosis and axon-specific pruning
(a) Wild-type (WT) sympathetic neurons (5 DIV) were treated with the following conditions to induce axon degeneration, respectively: NGF deprivation (-NGF), 20 μM etoposide (DNA Damage), 2.5 μM tunicamycin (endoplasmic reticulum (ER) Stress) and 1 μM vinblastine (to induce microtubule destabilization). NGF-maintained neurons served as controls. Neurons were fixed and immunostained for tubulin upon axon beading and fragmentation. (b) Neurons were treated as described in a and immunostained for cleaved Casp3 and cleaved Casp6. Nuclei were labelled with Hoechst. Scale bar, 20 μm.
