Figure 2: Casp6 is required for axon degeneration during axon pruning but not apoptosis.
From: Distinct pathways mediate axon degeneration during apoptosis and axon-specific pruning
(a) Wild-type (WT) and Casp6-deficient (Casp6 KO) neurons (5 DIV) were NGF-deprived for 24 h. WT neurons maintained in NGF served as a control. Scale bar, 10 μm. (b) Microfluidic chamber model of global deprivation, which induces soma and axon degeneration and local deprivation, which results in axon-selective degeneration. (c) WT and Casp6-deficient neurons were NGF maintained, globally deprived or locally deprived. Neurons were immunostained for tubulin and nuclei were labelled with Hoechst. Scale bar, 50 μm. (d) Quantification of axon degeneration for conditions shown in c. Data represent the mean±s.e.m. (n=3). P-values were calculated using an unpaired t-test.
