Figure 7: Mature neurons inhibit apoptosis during global deprivation but degenerate axons during local deprivation.
From: Distinct pathways mediate axon degeneration during apoptosis and axon-specific pruning
(a) Young (5 DIV) and mature (28 DIV) sympathetic neurons were either NGF maintained or NGF deprived for 48 h. Scale bar, 20 μm. (b) The same axons of young and mature neurons were imaged every 24 h during local deprivation. NGF-maintained neurons at both ages served as controls. Scale bar, 50 μm. (c) Quantification of axon degeneration for conditions shown in b. Data represent the mean±s.e.m. (n=3). (d) Cleaved Casp6 immunostaining and corresponding phase images (inset) in the axon compartments of conditions shown in b. All P-values were calculated using an unpaired t-test.
