Figure 1: Collagen VI is an extracellular component of SC niche.
From: Collagen VI regulates satellite cell self-renewal and muscle regeneration
(a) Representative confocal image of immunofluorescence analysis for collagen VI (red), Pax7 (green) and laminin (light pink) in TA cross-sections from wild-type mice. The arrowhead points at one SC. Scale bar, 25 μm. (b) Representative confocal image of a freshly isolated wild-type EDL fiber, showing the immunolocalization of collagen VI (red) with respect to SC (green, arrowhead). BF, bright field. Scale bar, 10 μm. (c) Expression levels of Col6a1 and Col1a1 transcripts in quiescent (QSC) and activated (ASC) wild-type SCs and in wild-type muscle fibroblasts (FBL). Gapdh expression was used to calculate the relative expression of Col6a1 and Col1a1 transcripts. Data are shown as mean±s.e.m. of three independent replicates (**P<0.01; unequal variance Student’s t-test; n=8, each group). (d) Representative confocal z-stack images of immunofluorescence for collagen VI (light pink) and laminin (red) in TA cross-sections from wild-type mice under untreated conditions or 4 days, 7 days and 30 days after single cardiotoxin injury. Scale bar, 50 μm. (e) Representative views of immunofluorescence for collagen VI (red) and Pax7 (green) in wild-type TA 4 days after cardiotoxin injection. Nuclei were stained with Hoechst (blue). Scale bar, 50 μm.
