Figure 2: Lack of collagen VI affects SC maintenance and muscle regeneration in vivo.
From: Collagen VI regulates satellite cell self-renewal and muscle regeneration
(a) Left panel, haematoxylin-eosin staining of TA cross-sections from wild-type and Col6a1–/– mice 7 days after cardiotoxin injury. The arrowheads point at some newly forming myofibers. Scale bar, 25 μm. Right panel, ratio of SCs between Col6a1–/– and wild-type TA in untreated conditions (−) and 7 days after single injury (+). Data are shown as mean±s.e.m of three independent replicates (**P<0.01; unequal variance Student's t-test; n=6 animals, each group). (b) Left panel, haematoxylin-eosin staining of TA cross-sections from wild-type and Col6a1–/– mice 30 days after cardiotoxin injury. The arrowheads point at some newly forming myofibers. Scale bar, 50 μm. Right panel, ratio of SCs between Col6a1–/– and wild-type TA in untreated conditions (−) and 30 days after single injury (+). Data are shown as mean±s.e.m. of three independent replicates (**P<0.01; unequal variance Student’s t-test; n=6 animals, each group). (c) Haematoxylin-eosin and Azan staining of TA cross-sections from wild-type and Col6a1–/– mice 7 days and 30 days after double and triple injury. Arrowheads point at some newly forming myofibers, asterisks label some areas still in the degeneration phase, and the dotted region in the lower right panel show fibrotic tissue substitution stained by Azan. Scale bar, 50 μm. (d) Quantification of TA weight/body weight of wild type and Col6a1–/– mice in untreated conditions (−) and 30 days after single, double or triple injury. Data are shown as mean±s.e.m. of three independent replicates (*P<0.05; **P<0.01; unequal variance Student’s t-test; n=6–8 animals, each group). (e) Ratio of SCs between Col6a1–/– and wild-type TA in untreated conditions (−) and 30 days after double or triple injury. Data are shown as mean±s.e.m. of three independent replicates (**P<0.01; unequal variance Student’s t-test; n=6 animals, each group). WT, wild-type.
