Figure 2: Forced expression of Zscan4 enhances developmental potency of ES cells.
From: Zscan4 restores the developmental potency of embryonic stem cells
(a) Schematic presentation of experimental procedure. (b) Expression levels of Zscan4 (endogenous and ORF) in Dox+ and Dox− conditions in G418-selected clones. Error bars indicate s.e.m., n=3. (c) Forced expression of βgeo in Dox+ and Dox− conditions. (d) Induction of Stra8 and Tmem92 (Zscan4-downstream genes) in Dox+ condition. Error bars indicate s.e.m., n=3. (e) Forced expression of Zscan4 protein detected by immunohistochemistry. Scale bar, 100 μm. (f) Assessment of developmental potency of ES cells by 4N complementation assays. The number shown near each data point indicates the number of transferred blastocysts. D+ and D− at p33 indicate the results for cells treated with Dox+ and Dox− at p31, respectively.
