Table 1 Tetraploid (4N) complementation assays confirm the higher potency of ES cells expressing a Zscan4c–ERT2 fusion protein.
From: Zscan4 restores the developmental potency of embryonic stem cells
Cell line | Genetic background | No. of injected cells | Passage no. | No. of blastocysts transferred | No. of embryos at E13.5 (%) | No. of live embryos (%) |
|---|---|---|---|---|---|---|
V6.5 | C57BL/6 × 129/sv | 10~15 | 18–19 | 108 | 4 (4) | 3 (3) |
V6.5 ZERT2 #7 | 22 | 40 | 2 (5) | 1 (3) | ||
V6.5 ZERT2 #10 | 22 | 41 | 9 (22) | 9 (22) | ||
V6.5 ZERT2 #18 | 19 | 40 | 22 (55) | 17 (43) | ||
TA1 | C57BL/6 × 129/sv | 10~15 | 3 | 20 | 13 (65) | 12 (60) |
9 | 27 | 8 (30) | 7 (26) | |||
18 | 34 | 8 (24) | 6 (18) | |||
MC2 | C57BL/6 | 10~15 | 17 | 117 | 0 (0) | 0 (0) |
MC2 ZERT2 #2 | 17 | 45 | 3 (7) | 3 (7) | ||
MC2 ZERT2 #4 | 17 | 36 | 5 (14) | 4 (11) | ||
MC2 ZERT2 #6 | 17–18 | 88 | 15 (17) | 13 (15) | ||
MC2 ZERT2 #7 | 17 | 47 | 2 (4) | 2 (4) | ||
MC2 ZERT2 #10 | 17 | 54 | 6 (11) | 3 (6) | ||
MC2 ZE #18 | C57BL/6 | 10~15 | 12–13 | 123 | 0 (0) | 0 (0) |
