Table 2 Tetraploid (4N) complementation assays confirm the higher potency of ES cells expressing a Zscan4c–ERT2 fusion protein.

From: Zscan4 restores the developmental potency of embryonic stem cells

Cell line

No. of injected cells

Passage no.

No. of blastocysts transferred

No. of embryos at full term (%)

No. of live embryos (%)

V6.5

1

18

71

1 (1)

1 (1)

V6.5 #2

1

21

77

0 (0)

0 (0)

V6.5 ZERT2 #18

1

21

44

3 (7)

2 (5)

TA1

1

4

79

3 (4)

3 (4)

  1. Single ES cells were injected into 4N blastocysts.