Figure 7: Metabolic phenotype of global Gpr83 ko mice after HFD exposure.

Body weight (a), body composition (b,c), glucose tolerance (ipGTT, 1.5 g glucose per g body weight) (d), insulin sensitivity (ipITT) (e), as well as food intake (f,g) of Gpr83 ko and wt mice fed with a HFD. Hypothalamic expression pattern of genes related to food intake (h) plasma levels of acyl- and desacyl ghrelin (i), as well as measurement of energy expenditure (j,k), locomotor activity (l) and RQ (m) in Gpr83 ko and matched wt control mice fed with a HFD. Panels (a–h) and (j–m) and contain N=5–9 mice each genotype, Panel i contains N=7–16 mice each genotype. Data in panel (h) are normalized to housekeeping gene hypoxanthine phosphoribosyltransferase 1 (HPRT). *P<0.05, **P<0.01, ***P<0.001 based on unpaired two-tailed Student’s t-test (b,d,g,h,k) or two-way analysis of variance followed by Bonferroni post-hoc test (f). Data represent means±s.e.m.