Figure 6: Cross-linking allows direct comparison of the untreated cATPase with the dephosphorylated form.

Interactions determined by a comparative cross-linking procedure (top schematic). No change in the extent of cross-linking between the two forms of the enzyme is represented with a solid black line. A reduction in cross-linking in the dephosphoylated form is shown by a two- to fivefold reduction (orange), 5–10 (magenta), >10 (red). Subunit δ is shown as a schematic. Phosphosites are shown in yellow. Changes in interactions are observed between the α/β interfaces and their interactions with subunits I, II, γ, δ and the extended structure of ε. Interactions between ε and the membrane ring are not affected (black).