Figure 6: Exocyst component and t-SNARE mutants suggest roles in pathogenicity.

(a) Targeted deletions of the SEC5, EXO70 and SSO1 genes in the aggressive Chinese field isolate O-137 (WT) resulted in a significant reduction in pathogenicity on a fully susceptible rice cultivar YT-16 in whole plant spray inoculation assays at Kansas State University. Numbers of lesions formed and lesion sizes were both reduced. (b) Similar pathogenicity defects were observed after inoculation of mutants in the Guy11 (WT) background on rice cultivar CO-39 at the University of Exeter. These data are presented as a bar chart showing the frequency of lesions formed per 5 cm of CO-39 leaf surface (P<0.05 for all mutants; n=30 for each mutant; mean±s.d., three experiments).