Figure 7: t-SNARE mutants suggest a role in BIC development. | Nature Communications

Figure 7: t-SNARE mutants suggest a role in BIC development.

From: Two distinct secretion systems facilitate tissue invasion by the rice blast fungus Magnaporthe oryzae

Figure 7

(a) Δsso1 mutant showing inappropriate secretion of Pwl2:mCherry:NLS during plant infection. In addition to BICs in the expected location, second fluorescent foci occurred midway along the primary hyphae. Images left to right: bright-field; GFP (green); and mCherry (red). (b) Another infection site in which Pwl2:mCherry:NLS expressed by an Δsso1 mutant strain shows inappropriate secretion. Images clockwise from the upper left: bright-field; merged mCherry (red) and GFP (green); fluorescence intensity linescans for mCherry (red) and GFP (green) along the white arrow; single channel GFP or mCherry fluorescence shown as black and white inverse images. (c) Bas1:mRFP expressed by an Δsso1 mutant shows inappropriate secretion. Images left to right: merged bright-field and mRFP; mRFP fluorescence shown as black and white inverse image; and fluorescence intensity linescan along the white arrow. Rice nuclei are indicated by a white asterisk. Linescans shown are representative of wild type (n=20) and knockout mutants (n=20 for each). Scale bars, 10 μm.

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