Figure 4: Rab10 regulates surface expression of axonal membrane proteins.
From: Myosin Vb controls biogenesis of post-Golgi Rab10 carriers during axon development
(a) Immuno-EM analysis for subcellular localization of indicated proteins and Rab10 in the corpus callosum regions from P0 rat brain. Red arrows indicate immunogold particles (18 nm) for Rab10 signals associated with PPV-like vesicles which were also marked by IGFR, TrkB or VAMP4 (yellow arrows, 12 nm). Scale bars=200 nm. (b) DIV2 hippocampal neurons transfected with vehicle plasmid or that encodes siMYO5B or exon D were live-stained with IGFR antibody without penetration. Co-expressed GFP was used to normalize neurite volumes. Note the polarized surface localization of IGFR in control cells was lost in siMYO5B or exon D neurons. Scale bar=50 μm. (c) Quantification for the surface IGFR signals in various groups. Data are presented as mean±s.e.m, with value from control group normalized as 1. *P<0.05, **P<0.01, Student’s t-test (n=5 for each group).
