Figure 2: Quantitation of cis-interaction between dynein and Rab6a(Q72L) by FCCS at the periphery of DRG neurons. | Nature Communications

Figure 2: Quantitation of cis-interaction between dynein and Rab6a(Q72L) by FCCS at the periphery of DRG neurons.

From: Rab6a releases LIS1 from a dynein idling complex and activates dynein for retrograde movement

Figure 2

The inset is a schematic diagram demonstrating fluorescent-labelled proteins used with each experiment. Auto- and cross-correlation curves of fluorescent-labelled proteins were shown for EGFP and TMR-HaloTag (a), EGFP-DIC1 and TMR-HaloTag-Rab6a(T27N) (b), EGFP-DIC1 and TMR-HaloTag-Rab6a(Q72L) (c), and EGFP-DIC1 and TMR-HaloTag-LIS1 (d). (e) Statistics of FCCS data: the values of RCA were calculated and plotted. P-values were calculated using analysis of variance (**P<0.01, ***P<0.001). All data were expressed as mean±s.d. N.S., not significant.

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