Figure 3: Demonstration of co-migration of dynein and Rab6a(Q72L) at the periphery of DRG neurons by TIRF.
From: Rab6a releases LIS1 from a dynein idling complex and activates dynein for retrograde movement

(a) Representative traces in the DRG neuorns by TIRF microscopy expressed with: EGFP-DIC1 and TMR-HaloTag-Rab6a(Q72L) (left panel), EGFP-DIC1 and TMR-HaloTag-Rab6a(T27N) (middle panel), and EGFP-DIC1 and TMR-HaloTag-LIS1 (right panels). White arrows indicate the direction towards the soma. The direction of transport was defined by the orientation of transport. Note: TMR-HaloTag-Rab6a(Q72L) co-migrated with EGFP-DIC1 in a retrograde fashion, whereas TMR-HaloTag-LIS1 co-migrated with EGFP-DIC1 in an anterograde fashion. TMR-HaloTag-Rab6a(T27N) did not display obvious co-migration. Scale bar, 5 μm. (b) MSDs were calculated from the motility of: EGFP-DIC1 and TMR-HaloTag-Rab6a(Q72L) (left panel); EGFP-DIC1 (second panel from the left); and EGFP-DIC1 and TMR-HaloTag-LIS1 (right two panels). Fits represent MSD ρ(Δt)=2DΔt+ν2Δt2. Note: traces of EGFP-DIC1, TMR-HaloTag-Rab6a(Q72L) and TMR-HaloTag-LIS1 fit second-order polynomial MSD (Δt), indicating that they are following ballistic scaling behaviour, rather than diffusive scaling behaviour.