Figure 4: LIS1 release from a LIS–dynein complex by Rab6a(Q72L), and the activation of dynein motility on MTs. Single-molecule movement of dynein, LIS1 and Rab6a(Q72L) coupled to Q-dots. | Nature Communications

Figure 4: LIS1 release from a LIS–dynein complex by Rab6a(Q72L), and the activation of dynein motility on MTs. Single-molecule movement of dynein, LIS1 and Rab6a(Q72L) coupled to Q-dots.

From: Rab6a releases LIS1 from a dynein idling complex and activates dynein for retrograde movement

Figure 4

(a) TIRF microscopic images of Q-dot-conjugated 605 Q-dot dynein (λ (emission)=605 nm, green), 525 Q-dot LIS1 (λ (emission)=525 nm, blue) and 655 Q-dot Rab6a(Q72L) (λ (emission)=655, red) are shown. The green, blue and red arrowheads indicate dynein, LIS1 and Rab6a(Q72L), respectively. Schematic combinations are shown at the right side. Rab6a(Q72L) facilitated dissociation of LIS1 from the LIS1–dynein idling complex. Released LIS1 disappeared. Scale bar, 1.0 μm. (b) The kymograph patterns of dynein (green line), Rab6a(Q72L) (red line) and LIS1 (blue line) are shown. Vertical scale bar, 1.0 μm; Horizontal scale bar, 1.0 s.

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