Figure 5: Knockdown of Rab6a results in an abnormal localization of dynein at the periphery of the DRGs. Down regulation of Rab6a by siRNA in DRG cells.
From: Rab6a releases LIS1 from a dynein idling complex and activates dynein for retrograde movement
(a) Western blotting analysis of Rab6a 48 h (or 0 h) after transfection with siRNA-targeting mouse Rab6a or pGL2 (left panel). Quantitation of normalized immunoblot values (right panel). P-values were calculated using a Student’s t-test (*P<0.05 and ***P<0.001). All data are expressed as mean±s.e.m. (b) Immunocytochemistry data 48 h after transfection with each of the siRNAs. Endogenous expression of Rab6a and DIC in the DRG neurons using the specific antibody for Rab6a and DIC. Note: Depletion of Rab6a induced an abnormal localization and accumulation of dynein at the periphery of the DRG cells. Scale bar, 10 μm. (c) Each percentage of aberrant distribution of dynein was presented, which was preferentially observed in Rab6a-depleted DRG neurons. N indicates the number of examined DRG neurons with independent four experiments. P-values were calculated using a Student’s t-test (***P<0.001). All data are expressed as mean±s.e.m. DAPI, 4',6-diamidino-2-phenylindole; N.S., not significant.
