Figure 2: The combination of contextual sequence and position determines the preference of phosphotyrosines for cis-interaction. | Nature Communications

Figure 2: The combination of contextual sequence and position determines the preference of phosphotyrosines for cis-interaction.

From: Antagonism between binding site affinity and conformational dynamics tunes alternative cis-interactions within Shp2

Figure 2

(a) The models depict the cis-interactions of the Y580F, Δ580 and Δ542 reporters upon phosphorylation in vitro. (b) The ratio time courses of Δ542 and Δ580 reporters. Y542 and Y580 sequences are coloured in red and green, respectively. (c) The binding curves of the C-SH2 domain (4.5 μM) towards immobilized pY542 or pY580 peptides measured by SPR (refer to Supplementary Fig. S8 for the binding kinetics and original sensorgrams). (d) The ratio time courses of engineered Y542Y542 and its mutant reporters as indicated.

Back to article page