Figure 5: Grb2 tunes the cis-interaction of Shp2 in MEFs. | Nature Communications

Figure 5: Grb2 tunes the cis-interaction of Shp2 in MEFs.

From: Antagonism between binding site affinity and conformational dynamics tunes alternative cis-interactions within Shp2

Figure 5

(a–c) The averaged ratios of the Y542F (a,b) or WT (c) reporter in MEFs coexpressing Tel-PDGFRβ with control vector, different amounts of Grb2 (a,c), or Grb2RV (b). (a) n=16, 17, 14, 18, 11, *, P<4.55e−5; (b) n=38, 25, 26, *, P<1.0e−8; (c) n=10, 20, 18, 15, 35, * P<3.1e−4 (Student’s t-test with Bonferroni correction). (d–e) The amount of Grb2 coimmunoprecipitated with WT or its mutant reporters in MEFs before and after 15 min PDGF stimulation. *, P<0.05 (Student’s t-test, n=3). Bar graphs represent the mean±s.e.m. of normalized intensity against its corresponding control group (refer to Supplementary Fig. S7a for the whole blots). (f) The model depicts the early interaction of C-SH2/pY542, the subsequent pY580 phosphorylation which recruits Grb2 and is sequestered by Grb2 to stabilize this C-SH2/pY542 pair.

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