Figure 6: The loss of tunable cis-interaction within the SWAP reporter and its effect on ERK phosphorylation. | Nature Communications

Figure 6: The loss of tunable cis-interaction within the SWAP reporter and its effect on ERK phosphorylation.

From: Antagonism between binding site affinity and conformational dynamics tunes alternative cis-interactions within Shp2

Figure 6

(a) Schematic drawings and comparison of the WT and SWAP Shp2 reporters. Y542 and Y580 sequences are coloured in red and green, respectively. (b) The phosphorylation of SWAP and its mutant reporters in vitro (left) or in MEFs (right, whole-cell lysate). (c,d) The ratio changes of Y542’FSWAP and Y580’FSWAP reporters (c) in vitro or (d) in MEFs. (e) The ERK phosphorylation at different time points (left) and its quantification (mean±s.e.m.) at 20 min (right) upon PDGF stimulation in MEFs expressing either WT or SWAP reporter as indicated. *, P<0.05 (Student’s t-test, n=3, refer to Supplementary Fig. S7b for the whole blots).

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