Figure 4: Analysis of 3D7-PDH+ and 3D7-OxoDH+ transgenic parasite lines.
From: Chemical and genetic validation of thiamine utilization as an antimalarial drug target
(a) Western blot analysis of transgenic parasites using monoclonal anti-strep antibodies. (b) Antiplasmodial activity of oxythiamine against P. falciparum 3D7-Mock (filled circles), 3D7-OxoDH+ (open squares) and 3D7-PDH+ (filled triangles) lines in thiamine-replete culture medium. (c) Antiplasmodial activity of oxythiamine against P. falciparum 3D7-Mock (filled circles), 3D7-OxoDH+ (open squares) and 3D7-PDH+ (filled triangles) lines in thiamine-free culture medium. The data are averaged from at least three independent experiments, each performed in duplicate and showed ±s.e.m. Where not shown, error bars are smaller than the symbols.
