Figure 7: Localization of PfPDH and PfOxoDH within erythrocytes infected with 3D7 parasites.
From: Chemical and genetic validation of thiamine utilization as an antimalarial drug target
(a) Western blot analysis of transgenic parasites expressing GFP-tagged leader sequences of PfPDH and PfOxoDH using monoclonal anti-GFP antibody. Wild-type 3D7 parasites are included as a control. (b) Localization of PfPDH (top panel) and PfOxoDH (bottom panel) in transgenic parasites. The left column shows phase contrast images, followed by fluorescence images of a nuclear dye (Hoechst; blue), GFP-tagged leader sequences of the target enzymes (green), a mitochondrial dye (MitoTracker; red) and all four images merged together (right column). Scale bars, 2 μm.
