Figure 1: Identification of the spontaneous Δ6PEST deletion mutant in the mouse 129 strains. | Nature Communications

Figure 1: Identification of the spontaneous Δ6PEST deletion mutant in the mouse 129 strains.

From: A spontaneous Cdt1 mutation in 129 mouse strains reveals a regulatory domain restraining replication licensing

Figure 1

(a) Lysates of exponentially growing mouse P19 and CGR8 cells were assessed by immunoblotting for expression of the indicated replication factors. The mutated form of Cdt1 expressed in the CGR8 ES cell line is indicated by an arrowhead. The asterisk indicates a non-specific band. (b) Cdt1 cDNA derived from CGR8 cells was cloned and sequenced. The homozygous mutation of Cdt1 consists in an 18-nucleotide deletion leading to a six-amino acid in-frame deletion within the PEST domain (Δ6PEST). The duplicated sequence found in the WT Cdt1 PEST domain is underlined. (c) PCR analysis of genomic DNA from P19, CGR8, D3, R1 and NIH 3T3 mouse cells using primers flanking the deleted region. PCR products corresponding to the WT and Δ6PEST (Δ6) alleles were separated on agarose gels. The size of the products is indicated.

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