Figure 3: Kir2.1 expression increases dendritic dynamics in ventral motoneurons.
From: Homeostatic regulation of dendritic dynamics in a motor map in vivo
(a,b) Images of a ventral motoneuron expressing Kir2.1-viral2a-mGFP at the first two consecutive time points (0 and 5 min). (c) Overlaid image of a and b. Arrows point to filopodial extensions. (d) Reconstruction (in white) of dendritic extensions over a 30-min period. (e,f) Images of a dorsal motoneuron at the first two consecutive time points (0 and 5 min). (g) Overlaid image of e,f. Arrows point to filopodial extensions. (h) Reconstruction (in white) of filopodial extensions over a 30-min period. (i,j) Quantification of filopodial extensions and retractions between consecutive time points for 16 Kir2.1-viral2a-mGFP expressing motoneurons. Each row represents a single cell and the rows are stacked from bottom to top based on the ventral to dorsal position of the cell in the spinal cord. Each column represents the total length of filopodial extensions between two consecutive time points normalized to the dendritic arbor length of that cell. Colour map is on the right. Scale bar, 10 μm in a–h.
