Figure 2: S. aureus priming attenuates lung injury mediated by influenza infection.

(a) Change in body weight of control WT mice and S. aureus-primed WT mice at the indicated times after infecting with a sublethal dose (0.1 HA) of PR8. (b) Hematoxylin and eosin staining of lung, (c)inflammation scores and (d) BCA protein assay of total protein in BALF from control WT mice and S. aureus-primed WT mice at the indicated times after infecting with 0.1 HA of PR8. Scale bars represent 200 μm. (e) ELISA of cytokines and chemokines in BALF from control and S. aureus-primed WT mice at day 7 after infecting with 0.1 HA of PR8. Two-tailed Student’s t-tests, *P<0.05, **P<0.01, ***P<0.001. Data are expressed as mean±s.e.m. Data represent three independent experiments with at least five mice per group in (a–e).