Figure 5: The number of bipolar subapically dividing RGs is increased in Trnp1-shRNA electroporated cells in the developing mouse cerebral cortex.
From: Amplification of progenitors in the mammalian telencephalon includes a new radial glial cell type
(a) Fluorescent micrograph of a section of embryonic day 14 cerebral cortex 24 h after in utero electroporation with either control plasmid (left) or Trnp1-shRNA-GFP plasmid (right). Note the expanded DAPI-dense OSVZ-like region (indicated by the white line in the third panel) with many proliferating (PH3+) cells (indicated by white arrows). (b) Subapical RGs after Trnp1 knockdown identified by PH3 (red) and bipolar morphology in M-phase (white arrows pointing to green, GFP-labelled processes). (c) Quantification of bipolar-GFP+/PH3+ RGs dividing at non-apical positions among all GFP+/PH3+ cells after Trnp1 knockdown in comparison with the control (control n=159 cells; Trnp1-shRNA n=203 cells, three animals in each condition; error bars are s.e.m.). (d) Still frames of a movie of a subapically dividing RG imaged 48 h after Trnp1-shRNA electroporation. Note the characteristic apically directed movement before dividing at subapical position (arrows point to cell somata, arrowheads towards the long basal process). Scale bars 100 μm (a,b), 20 μm (d).
